This is achieved with a spectrophotometer. It is important to measure the quality and quantity of extracted DNA. In a new tube add 2 ul of your extracted DNA and the calculated amount of molecular grade water, label tube with lab ID and a capital D to indicate it is diluted. This time to find out how much molecular grade water we need to add, we subtract the 2 ul of DNA template from the Final volume. If not use the calculation below.įor DNA concentrations higher than 100 ng/ul There should be enough room to add the molecular grade water in the microfuge tube holding your DNA sample. ** To find out how much molecular grade water we need to add we subtract the amount of EB we used from Final volume. V1 – V2 = the amount of liquid you will add **.V2 = The total volume we will have after diluting.C2 = 20 ng/ul this is the concentration we want.V1 = The total amount of elution buffer (EB) you used.C1 = Concentration of DNA measured (ng/ul).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |